Accurately Measure Cholesterol with Amplex Red Cholesterol Assay Kit: A Reliable Solution for Research

Measure cholesterol with ease using the Amplex Red Cholesterol Assay Kit. It's fast, sensitive, and reliable.

The Amplex Red Cholesterol Assay Kit is a cutting-edge tool for measuring cholesterol levels in biological samples. With its advanced technology and precise measurements, this kit is revolutionizing the field of lipid research. Through a series of carefully designed steps, the assay kit quantifies cholesterol levels with accuracy and reliability, providing scientists with a powerful tool to investigate the role of cholesterol in various diseases and conditions.

Furthermore, this innovative kit features an easy-to-use protocol that is suitable for both novice and experienced researchers. The assay process is streamlined and efficient, allowing for high-throughput analysis of multiple samples in a short amount of time. Whether you are studying cardiovascular disease, metabolic disorders, or other health-related issues, the Amplex Red Cholesterol Assay Kit is an essential resource for any researcher looking to gain insight into the complex world of lipids and cholesterol.

Not only is this assay kit highly effective, but it is also cost-effective, making it an ideal choice for laboratories on a budget. With its reliable results and user-friendly design, the Amplex Red Cholesterol Assay Kit is quickly becoming the go-to tool for lipid researchers around the world.

Introduction

The Amplex Red Cholesterol Assay Kit is a highly sensitive and reliable method for measuring cholesterol levels in biological samples. The kit has been designed to detect both free and esterified cholesterol, making it suitable for use in a wide range of applications, from basic research to clinical diagnostics.

Principle of the assay

The Amplex Red Cholesterol Assay Kit is based on the enzymatic oxidation of cholesterol by cholesterol oxidase, which produces hydrogen peroxide (H₂O₂) as a byproduct. In the presence of horseradish peroxidase (HRP) and Amplex Red reagent, H₂O₂ reacts with Amplex Red to produce a fluorescent product that can be measured using a fluorometer or a microplate reader.

Advantages of the kit

The Amplex Red Cholesterol Assay Kit offers several advantages over other cholesterol detection methods:

• High sensitivity: the kit can detect cholesterol levels as low as 10 µM
• Wide linear range: the kit can measure cholesterol concentrations from 0.1 to 100 µM
• High specificity: the kit can distinguish between free and esterified cholesterol
• Easy to use: the kit requires minimal sample preparation and can be completed in less than 2 hours

Applications of the kit

The Amplex Red Cholesterol Assay Kit has been used in a variety of applications, including:

• Studying the role of cholesterol in cellular metabolism and signaling pathways
• Assessing the efficacy of cholesterol-lowering drugs and other interventions
• Monitoring cholesterol levels in patients with cardiovascular disease or other metabolic disorders
• Detecting cholesterol in food products and environmental samples

Sample preparation

The Amplex Red Cholesterol Assay Kit can be used with a variety of biological samples, including serum, plasma, cell lysates, and tissue homogenates. Sample preparation typically involves extraction of lipids using a chloroform/methanol mixture, followed by evaporation and reconstitution in assay buffer.

Assay procedure

The Amplex Red Cholesterol Assay Kit can be performed in a 96-well plate format, with each well containing 50 µl of sample and 50 µl of reaction mixture. After a brief incubation period, fluorescence is measured using a fluorometer or a microplate reader. The kit also includes a cholesterol standard for calibration.

Assay validation

The Amplex Red Cholesterol Assay Kit has been validated for accuracy and precision by several research groups. In one study, the kit was compared to a commercially available enzymatic cholesterol assay kit and showed excellent correlation with the reference method (r = 0.99).

Storage and stability

The Amplex Red Cholesterol Assay Kit should be stored at -20°C and protected from light. The kit components are stable for at least 1 year when stored properly.

Conclusion

The Amplex Red Cholesterol Assay Kit is a highly sensitive and reliable method for measuring cholesterol levels in biological samples. Its high specificity, wide linear range, and ease of use make it suitable for a variety of applications in basic research and clinical diagnostics.

Amplex Red Cholesterol Assay Kit: Accurate and Specific Quantification of Cholesterol

The Amplex Red Cholesterol Assay Kit is a reliable and sensitive tool for measuring cholesterol levels in biological samples. The principle of the assay is based on the enzymatic oxidation of cholesterol by cholesterol oxidase to produce hydrogen peroxide and cholest-4-en-3-one. The generated hydrogen peroxide is then detected using Amplex Red reagent in the presence of horseradish peroxidase (HRP).

Sensitivity and Specificity

The assay has a high sensitivity, capable of detecting cholesterol concentrations as low as 0.05 mM in biological samples. Additionally, the Amplex Red Cholesterol Assay Kit is highly specific to cholesterol, making it a dependable tool for measuring cholesterol levels without interference from other sterols or non-cholesterol sterols.

Sample Compatibility and Components

This assay can be performed on a wide variety of samples, including serum, plasma, tissue homogenates, and cultured cells. All necessary reagents (enzyme mix, reaction buffer, Amplex Red reagent, HRP, and cholesterol standard) for performing 100 assays are included in the kit.

Standard Curve and Detection Method

The kit includes a cholesterol standard to generate a standard curve and quantify the amount of cholesterol in the unknown sample. The assay is performed using a fluorometric detection method, which provides a more sensitive and accurate measurement than colorimetric methods.

Time-Saving and Shelf-Life

The Amplex Red Cholesterol Assay Kit requires only a few steps, and the reaction can be completed in less than 30 minutes, saving valuable time in experiments. Additionally, the kit components have a long shelf-life, ensuring repeated use over a prolonged period.

Research and Clinical Applications

The Amplex Red Cholesterol Assay Kit is commonly used in research and clinical studies to quantify cholesterol levels in various biological samples. The accurate measurement of cholesterol levels can aid in understanding the pathophysiology of cholesterol-related diseases such as atherosclerosis, coronary heart disease, and obesity.

In conclusion, the Amplex Red Cholesterol Assay Kit provides an accurate, sensitive, and specific method for quantifying cholesterol in various biological samples. Its ease of use, time-saving assay, and long shelf-life make it a reliable tool for research and clinical applications.

Once upon a time, there was a revolutionary product in the world of biochemistry called the Amplex Red Cholesterol Assay Kit. This kit was designed to measure cholesterol levels in biological samples with a high degree of accuracy and precision. It was developed by a team of experts who understood the importance of cholesterol measurement in various fields such as clinical research, drug discovery, and food science.

The Amplex Red Cholesterol Assay Kit works by using a unique enzyme-based reaction that converts cholesterol into a fluorescent compound. This compound can then be easily detected using a fluorescence reader, which provides highly sensitive and reliable results. The kit is also very easy to use, even for those who have little experience in biochemistry or laboratory work.

The Amplex Red Cholesterol Assay Kit has many benefits, including:

1. High sensitivity: The kit can detect cholesterol levels as low as 0.1 μM, making it ideal for detecting small changes in cholesterol levels that may be missed by other methods.
2. Wide dynamic range: The kit can measure cholesterol levels between 0.1 μM and 10 μM, allowing for accurate measurements in both low and high cholesterol samples.
3. Easy to use: The kit comes with all the necessary reagents and detailed instructions, making it easy to use even for those with little experience in biochemistry.
4. Reliable results: The kit provides highly reproducible results, ensuring that measurements are consistent across multiple experiments and users.

Overall, the Amplex Red Cholesterol Assay Kit is an essential tool for anyone working in the field of biochemistry or related areas. Its ease of use, high sensitivity, wide dynamic range, and reliable results make it an indispensable product for measuring cholesterol levels in biological samples.

Thank you for taking the time to read our article about the Amplex Red Cholesterol Assay Kit. We hope that we have provided you with a comprehensive overview of this powerful tool for measuring cholesterol levels in biological samples. As we have discussed, the Amplex Red Cholesterol Assay Kit offers many advantages over other methods of cholesterol detection, including high sensitivity, low interference, and ease of use.

Whether you are a researcher, a clinician, or a laboratory technician, the Amplex Red Cholesterol Assay Kit can provide you with accurate and reliable data on cholesterol levels in a wide range of sample types. By choosing this kit, you can be confident that you are using a proven method that has been validated in numerous published studies.

In conclusion, we highly recommend the Amplex Red Cholesterol Assay Kit as a valuable tool for anyone who needs to measure cholesterol levels in biological samples. Its superior performance, ease of use, and versatility make it an ideal choice for a wide range of applications. We invite you to learn more about this kit and to consider using it in your next experiment or clinical study. Thank you again for visiting our blog, and we wish you success in all of your research endeavors!

People also ask about Amplex Red Cholesterol Assay Kit:

1. What is the Amplex Red Cholesterol Assay Kit?

   The Amplex Red Cholesterol Assay Kit is a highly sensitive and easy-to-use assay kit for the quantification of cholesterol in various biological samples.

2. How does the Amplex Red Cholesterol Assay Kit work?

   The Amplex Red Cholesterol Assay Kit uses a two-step enzymatic reaction to measure cholesterol levels. In the first step, cholesterol esterase hydrolyzes cholesterol esters to free cholesterol and fatty acids. In the second step, cholesterol oxidase converts free cholesterol to cholest-4-en-3-one and hydrogen peroxide. The Amplex Red reagent reacts with hydrogen peroxide in the presence of horseradish peroxidase (HRP) to produce a fluorescent product, resorufin, which can be detected using a fluorescence plate reader or a fluorescence microscope.

3. What are the advantages of using the Amplex Red Cholesterol Assay Kit?

   The advantages of using the Amplex Red Cholesterol Assay Kit include:

   • High sensitivity: The kit can detect as low as 10 µM cholesterol in biological samples.
   • Wide linear range: The kit has a linear range of 0.1-10 mM cholesterol.
   • Easy-to-use: The kit requires only small sample volumes and can be completed within 30 minutes.
   • Compatibility: The kit can be used with various biological samples, such as serum, plasma, cell lysates, and tissue homogenates.

4. What are the applications of the Amplex Red Cholesterol Assay Kit?

   The applications of the Amplex Red Cholesterol Assay Kit include:

   • Quantification of cholesterol in biological samples for research purposes
   • Drug discovery and development for hypercholesterolemia treatment
   • Screening for cholesterol-lowering agents
   • Detection of cholesterol in food and beverages

5. What is included in the Amplex Red Cholesterol Assay Kit?

   The Amplex Red Cholesterol Assay Kit includes:

   • Cholesterol esterase
   • Cholesterol oxidase
   • Amplex Red reagent
   • HRP
   • Assay buffer
   • Cholesterol standard
   • Instruction manual